A Complete Pipette Guide

Certainly! Here’s a complete guide to pipette, including their types, usage, maintenance, and common troubleshooting tips:

  1. What is a Pipette?

A pipette is a laboratory instrument used to accurately measure and transfer small volumes of liquid. It is commonly used in scientific research, medical diagnostics, and various other applications.

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  1. Types of Pipettes:
  2. Volumetric Pipettes: These pipettes are used to measure a specific volume of liquid precisely. They have a single graduation mark and are commonly available in capacities of 1 mL, 2 mL, 5 mL, and so on.
  3. Mohr Pipettes: These pipettes are similar to volumetric pipettes but have multiple graduation marks. They are commonly used for delivering variable volumes of liquids.
  4. Serological Pipettes: These pipettes have a long, cylindrical shape and are primarily used in cell culture and microbiology work. They have graduation marks along their length and can deliver various volumes.
  5. Micropipettes: Micropipettes are the most commonly used pipettes in laboratories. They are adjustable and can accurately measure and transfer microliter volumes. Micropipettes are further categorized into:

      – Single-channel pipettes: These pipettes can handle one sample at a time.

– Multi-channel pipettes: These pipettes have multiple channels, typically 8 or 12, allowing simultaneous pipetting into multiple wells or tubes.

  1. Usage of Pipettes:
  2. Preparing the Pipette: Before using a pipette, ensure it is clean and calibrated. Select the appropriate pipette tip for the desired volume range.
  3. Setting the Volume: Adjust the volume on the pipette by rotating the plunger or using the digital display (in electronic pipettes).
  4. Pipetting Technique:

      – For volumetric pipettes: Immerse the pipette tip into the liquid, then draw the liquid up to slightly above the graduation mark. To dispense, touch the tip to the receiving vessel and gently release the liquid.

      – For micropipettes: Depress the plunger to the first stop to draw the liquid, immerse the tip in the liquid, and slowly release the plunger to dispense the liquid.

  1. Avoiding Contamination: Change the pipette tip for each new sample to prevent cross-contamination.
  2. Cleaning after Use: Clean the pipette by wiping the exterior with a mild disinfectant. Check the manufacturer’s instructions for specific cleaning guidelines.
  3. Pipette Maintenance:
  4. Calibration: Regularly calibrate pipettes to ensure accurate measurements. Consult the manufacturer’s guidelines or hire a professional service for calibration.
  5. Plunger Lubrication: Some pipettes may require periodic lubrication of the plunger mechanism. Follow the manufacturer’s instructions for lubrication intervals and methods.
  6. Storage: Store pipettes in an upright position in a clean and dry location. Avoid subjecting them to extreme temperatures or mechanical shocks.
  7. Service and Repair: If a pipette is malfunctioning or damaged, contact the manufacturer or a certified service provider for repairs or maintenance.
  8. Troubleshooting Common Issues:
  9. Inaccuracy: Ensure proper calibration and technique while pipetting. Check the pipette tip for damage or clogging.
  10. Leakage: Inspect the pipette for cracks or damaged seals. Ensure the pipette tip is properly attached.
  11. Difficult Plunger Movement: Clean the pipette and lubricate the plunger if necessary.
  12. Tip Ejection Issues: Check for tip ejection mechanism damage and ensure the tip is properly attached.

Remember to consult the specific user manual provided by the manufacturer for detailed instructions and guidelines on using and maintaining your specific pipette model.

Omsons Glassware is a manufacturer and exporter of laboratory glassware products based in India. They offer a wide range of lab glassware products, including beakers, flasks, pipettes, burettes, funnels, and more.

If you are looking to purchase Omsons Glassware products, you can visit their website or contact them directly to inquire about purchasing options. They may have distributors or suppliers in your region or be able to ship products to you directly. Additionally, there may be other laboratory glassware suppliers in your region that offer similar products and services, so it is worth researching and comparing different suppliers to find the best options for your specific needs.

Note: The information provided here is a general guide. Always refer to the manufacturer’s instructions and follow the best practices specific to your pipette model for accurate and safe usage.


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